Features that vary in a diel pattern are ubiquitous among different organisms. Regarding scleractinian corals, physiological traits are known to change between day and night, but knowledge on oscillations in their associated microbiome is incipient. Currently available data is based on a few species of zooxanthellate corals (i.e. corals that host photosynthetic dinoflagellates), majoritarily from the Pacific Ocean. In an effort to expand the current understanding of the dial variation in coral microbiome, we investigated their composition and abundance during a 72-hour period for four species: the zooxanthellate Mussismilia hispida and Madracis decactis, and the azooxanthellate Tubastraea coccinea and T. tagusensis. Samples were taken every four hours for three days (each day corresponding to a replicate) in an experiment set in the Alcatrazes Archipelago (Southwestern Atlantic). After Illumina sequencing of 16S rDNA, DADA2 was used for quality control and taxonomy assignment. Data were rarefied and statistical analyses were performed in R and are still in progress. Alpha diversity (Shannon) was significantly different between M. decactis and the other species but not between daytime and nighttime within each species. Beta diversity (Bray-Curtis/PERMANOVA) by species followed a similar pattern, with no difference between day and night or by time. Regarding diel variation, calculated by JTKcycle for each species, significant results (ADJ.P<0.01) were found for one ASV (Amplicon Sequence Variant) in T. tagusensis, corresponding to Pseudoalteromonas sp. (highest abundance at 4 am). A lack of overall diel variation can suggest that these coral hosts have a greater ability to regulate their microbial communities. ASVs with diel rhythmicity were previously found in a zooxanthellate coral and an azooxanthellate anemone but while none of them belonged to Pseudoalteromonas, some belonged to the same class (Gammaproteobacteria). Future analyses should refine our current understanding of variations in the coral microbiome.
Ecologia e Fisiologia - Comunicações orais
Diel rhythms in coral microbial communities
Forma de apresentação: Oral
Seiblitz, Isabela G. L. (1)(2); Capel, Kátia C. C. (1)(3); Zanotti, Aline A. (1)(4); Zilberberg, Carla (5)(6); Cordeiro, Cesar A. M. M. (7); Oliveira, Raquel R. (8); Francini, Carlo Leopoldo B. (9); Garrido, Amana G. (1)(6); Kitahara, Marcelo V. (1)
(1) Centro de Biologia Marinha da Universidade de São Paulo, CEBIMar/USP, São Sebastião, SP. (2) Programa de Pós-Graduação em Zoologia, Instituto de Biociências da Universidade de São Paulo, IB/USP, São Paulo, SP. (3) Museu Nacional da Universidade Federal do Rio de Janeiro, MN/UFRJ, Rio de Janeiro, RJ. (4) Programa de Pós Graduação em Sistemas Costeiros e Oceânicos, Universidade Federal do Paraná, PGSISCO/UFPR, Pontal do Paraná, PR. (5) Instituto de Biodiversidade e Sustentabilidade da Universidade Federal do Rio de Janeiro, NUPEM-UFRJ, Macaé, RJ. (6) Instituto Coral Vivo, Rio de Janeiro, RJ. (7) Laboratório de Ciências Ambientais da Universidade Estadual do Norte Fluminense, UENF, Campos dos Goytacazes, RJ. (8) Faculdade de Oceanografia da Universidade do Estado do Rio de Janeiro, FAOC/UERJ, Rio de Janeiro, RJ. (9) Instituto Laje Viva, Santos, SP.
Autor que fará a apresentação: Seiblitz, Isabela G. L.
Email do autor que fará a apresentação: isaseiblitz@gmail.com
Financiamento: Fundação de Amparo à Pesquisa do Estado de São Paulo (Fapesp)
O trabalho foi desenvolvido com o uso da infraestrutura do CEBIMar? Sim