DNA barcode has risen as an effective method for investigating and revising taxonomic diversity, including the identification of cryptic species. Cymadusa filosa Savigny, 1816 (Crustacea: Amphipoda) has long been considered a species complex. Seven species from the genus Cymadusa have been recorded for Brazilian coast, including Cymadusa tartarugae Andrade & Senna, 2017, in Armação dos Búzios, RJ. We recently discovered a population of Cymadusa in Palmas Island (Ubatuba, SP) that presented many diagnostic features of C. tartarugae, including the morphology of gnathopods. In this study, we investigated the species boundaries between C. filosa and C. tartarugae, using an integrative approach with morphological and molecular data. We included populations of C. tartarugae from Arraial do Cabo; C. filosa from Arraial do Cabo and different sites along SP coast, and Cymadusa cf. tartarugae from Palmas Island. We characterized the shape of the propodus of Gnathopods 1 and 2 with geometric morphometric analyses. We sequenced the barcoding region of COI gene and included sequences from other Cymadusa species available on public repositories. Available sequences of Ampithoe ramondi were used as an outgroup. Morphometric analysis supported the separation between C. filosa and C. tartarugae, with specimens from Palmas grouping with C. tartarugae. Molecular analysis confirms the existence of two separate valid species. However, Palmas population is genetically similar to C. filosa. Therefore, in this study we genetically validate C. tartarugae as a separate species from C. filosa. However, we reinforce the great morphological variability of C. filosa, with the existence of morphotypes that are cryptic to C. tartarugae. Our results highlight the importance of using integrative taxonomic approaches for species delimitation, given approaches based solely on morphology may lead to misidentifications. We encourage further studies for understanding the evolutionary origin and the ecological drivers of the revealed morphological congruence between C. filosa and C. tartarugae.
Sistemática e Evolução - Comunicações orais
Are morphological and molecular data enough to unveil species boundaries? the case of two congeneric amphipods.
Forma de apresentação: Oral
Longo, Pedro A. S. (1)(2); Siqueira, Silvana G. L. (3); Mansur, Karine F. R. (4); Azevedo-Silva, Marianne (2); Leite, Fosca P. P. (3).
(1) Laboratório de Ecologia e Conservação Marinha, Instituto do Mar, Universidade Federal de São Paulo, Santos, Brazil. (2) Programa de Pós-Graduação em Ecologia, Instituto de Biologia, Universidade Estadual de Campinas, C.P. 6109, 13083-862 Campinas, SP, Brazil. (3) Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, 13083-862, Brazil. (4) Programa de Pós-Graduação em Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, C.P. 6109, 13083-862 Campinas, SP, Brazil.
Autor que fará a apresentação: Longo, Pedro A. S.
Email do autor que fará a apresentação: pedro.slongo@gmail.com
Financiamento: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
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